Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: p-p65 Rabbit Ab , Bioss , bs-0982R , 1: 1500.

Techniques: Expressing

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: p-p65 Rabbit Ab , Bioss , bs-0982R , 1: 1500.

Techniques: Activity Assay

Journal: Materials Today Bio

Article Title: Ultrasmall Prussian blue–integrated cryogel for enhanced ROS scavenging and immunomodulation via cGAS–STING inhibition in wound healing

doi: 10.1016/j.mtbio.2026.103056

Figure Lengend Snippet: Potential therapeutic mechanism of Cryogel@USPB in wounds. (A) The relative mRNA expression of STING1 and interferon alpha after the treatment of Cryogel@USPB (n = 3). (B) Immunofluorescent staining of F4/80 (green), STING (red), and DAPI (blue) on day7 in diabetic wounds. (C) Immunofluorescent staining of F4/80 (green), STING (red), and DAPI (blue) on day7 in deep second-degree burn wounds. (D) Expression of STING, p-STING, cGAS, IRF3, p-IRF3, p65, P-p65 in three individual skin wound tissues of diabetic mice in the Cryogel@USPB and control group. (E) Expression of STING, p-STING, cGAS, IRF3, P-IRF3, p65, p-p65 in three individual skin tissues of deep second-degree burn wounds in the Cryogel@USPB and control group. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The primary antibodies were: cGAS (1:1000, Proteintech, USA), STING (1:1000, Proteintech, USA), p-STING (1:1000, Proteintech, USA), IRF3 (1:1000, CST, USA), p-IRF3(1:1000, CST, USA), p65(1:1000, CST, USA ) , p-p65(1:1000, CST, USA ) , GADPH (1:5000, CST, USA) and β-actin (1:5000, CST, USA).

Techniques: Expressing, Staining, Control

Journal: Oncology Letters

Article Title: Schisandrin B suppresses cholangiocarcinoma by targeting the ROS/p38 MAPK/NF-κB axis

doi: 10.3892/ol.2026.15551

Figure Lengend Snippet: Sch B inhibits NF-κB signaling pathway expression. mRNA expression levels of (A) IL-6, (B) IL-8 (C) and TNF-α were measured in CCA cells treated with different concentrations of Sch B (0, 40, 80, 160 µmol/l). Statistical analysis was performed using Welch's ANOVA followed by Dunnett's T3 post hoc test. (D) NF-κB expression in CCA cells was evaluated using a double luciferase assay with different concentrations of Sch B (0, 10, 20, 40, 80, 160 µmol/l). (E) Hoechst immunofluorescence staining of p65 expression in CCA cells. (F) CCA cell activity was assessed after treatment with Sch B and Bay 11–7082, a targeted NF-κB inhibitor. Statistical analysis was performed using ANOVA and Dunnett's post hoc test. *P<0.05, **P<0.01, ***P<0.001. Sch B, Schisandrin B; CCA, cholangiocarcinoma.

Article Snippet: p-p65 NF-κB (Ser536) , 3033 , 1:1,000 , Cell Signaling Technology, Inc..

Techniques: Expressing, Luciferase, Immunofluorescence, Staining, Activity Assay

Journal: Frontiers in Pharmacology

Article Title: Investigating the therapeutic mechanism of Puerarin in vascular dementia: an integrated approach combining network pharmacology and experimental validation

doi: 10.3389/fphar.2026.1796295

Figure Lengend Snippet: PUE inhibits the TLR4/MyD88/NF-κB signaling pathway in VaD rats. (A) mRNA levels of TLR4 and MyD88( n = 4); (B) Immunohistochemical staining of P- NF-κB P65 in hippocampus (200×, 400×); (C–F) Protein levels of TLR4, Myd88, P- NF-κB P65, NF-κB P65 ( n = 4); Data are presented as mean ± SEM. Significant differences compared with Sham group were designated as * P < 0.05, with Model group as # P < 0.05, with PUE group as & P < 0.05 and with LPS group as △ P < 0.05.

Article Snippet: Briefly, the sections were pretreated with sodium citrate buffer (Boster Biological Technology, Pleasanton, CA, USA, Cat. No.: AR0024) at 98 °C for 30 min for antigen retrieval, and then the endogenous peroxidase activity was blocked with SignalStain® Peroxidase Blocking Reagent (CST, Danvers, MA, USA, Cat. No.: 15039S) for 10 min and then with BSA (Biyuntian biological technology, Shanghai, China, Cat. No.: ST023) for 30 min, incubated with the primary P-NF-κB p65 (1:300, abmart, Shanghai, China, No.: TP56372) overnight at 4 °C, and finally with goat anti-mouse biotinylated antibody (1:200, CST, Danvers, MA, USA, No.: 14709S) for 30 min at room temperature.

Techniques: Immunohistochemical staining, Staining